NASC Stock Detail Page
NASC ID: N99335
Name: TFB-SCHP-4-7
ABRC stock number: CS99335
Description: This subpool (CS99335) consists of 90 independent transformants expressing artificial microRNAs (amiRNAs) designed to target homologous, possibly functionally redundant genes in forward genetic screens; represents a subpool of TFB amiRNA pool N99430. Note that the larger pool N99430 is recommended for forward genetic screening of many lines. Generated by transforming Col-0 plants with a combination of 20bp and 21bp amiRNA libraries (CD4-62 and CD4-72, respectively) in p35S::amiRNA3 OCS (pPHANTOM vector) targeting transcripts encoding transcription factors and other RNA and DNA binding proteins (TFB); includes protein families in TransfacDB; protein family member functional classes: DNA or RNA binding (GO:0003677; GO:0003723) and transcription factor activity (GO:0003700). Basta resistant.
1250 seeds provided per order.
Donation Date: 2017-10-26
| Donated by: | University of California, San Diego Julian Schroeder University of California, San Diego Felix Hauser |
Part of : amiRNA pool
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £30.00 |
Germplasm Info
Seed type: Transgenic
Background: Col(Columbia)
Growth requirement: Select on BASTA plates or with Finale for selection on soil
Phenotype
Mixture of phenotypes depending on unknown amiRNA expressed.
References
Hauser, F. et al. 2013. A genomic-scale artificial microRNA library as a tool to investigate the functionally redundant gene space in Arabidopsis. The Plant Cell 25(8):2848-63. PubMed ID: 23956262.
Hauser F, Ceciliato PHO, Lin YC, Guo D, Gregerson JD, Abbasi N, Youhanna D, Park J, Dubeaux G, Shani E, Poomchongkho N, Schroeder JI; 2018-10-20; A seed resource for screening functionally redundant genes and isolation of new mutants impaired in CO2 and ABA responses.; JOURNAL OF EXPERIMENTAL BOTANY; PubMed ID: 30346611